Molecular investigation of Echinococcus granulosus in infected patients in Babylon province

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Nadia Zaidan Khlaif Al-Nuaimi
Sahar Jaber Mohesn Al-Hassani


The current study which was conducted during the period from (1/5/2022 to 12/31/2022 ) aimed to
follow up people with echinococcosis in the hospitals of Babylon Governorate and some of its different
districts, which included Hilla Surgical Hospital, Imam Ali Hospital, Al Hashimiya Hospital, in
addition to ( Al-Salam, Babylon , Al-Hayat) for the purpose of determining the prevalence of hydatid
cyst worm by diagnosis by molecular investigation. The study included examining 94 blood samples
distributed into three groups of 32 blood samples for people infected with echinococcosis before the
hydatid cyst removal operation (12 males and 20 females). 32 blood samples for people after the
surgical operation and removal of hydatid cysts and within (3-6) months (21 males and 11 females) and
females) and 30 samples for the control group (healthiest) (17 males and 13 females). The clinical
examination was conducted by Consultant doctors as well as diagnosis by | X-ray, ultrasonography,
and tomography computer showed the results of the molecular study of hydatid cyst samples of
infected people after extracting DNA from the germinal layer and protoscolices of hydatid fluid, using
the multiplex polymerase chain reaction (PCR) technique and the tree phylogenetic tree. And based on
the diagnostic gene (cytochrome oxidase Col) with a size of bp446 nucleotide pair, and comparing the
similarity rates among the isolates registered globally and using the MEGA program, and comparing
them with the similarity rates in our current study with the local and global isolates registered in the
NCBI Global Gene Bank and within the base database and using the program NCBI where we were
provided with the universal sequence numbers of the isolate

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Author Biography

Nadia Zaidan Khlaif Al-Nuaimi

Ph.D-Student, Second Part of Thesis, Biology Department, Collage of Education for Girls, Kufa
University, Iraq