Micro propagation and evaluation of growth parameters of Bacopa monnieri (L)

Abstract

Bacopa monnieri is an important medicinal and economically useful plant. Its extracts are of primary ingredient in many pharmaceutical goods and are utilized for therapeutic purposes. Due to indiscriminate pharmaceutical demand, it has been obliterated from its natural environment. Therefore, an efficient protocol was developed for rapid micropropagation of Bacopa monnieri. In vitro multiplication method was used for the regeneration of this extremely endangered germplasm. Nodal and shoot tips were explanted on MS media with 3% sucrose, 0.8% agar, and different plant growth regulators. Shoot tip explants on MS media with 1.0 mg/l BAP + 0.25 mg/l Kinetin had the best responses. The best rooting response was on MS media with 1.0 mg/l IAA + 0.2 mg/l NAA.The regenerated and rooted plantlets were acclimatized and successfully transferred to soil for normal growth under field conditions with a 75-83% success rate after primary and secondary hardening. This procedure could be used to preserve and massively multiply economically significant and commercially valuable plants.