A Physiological Study for Evaluating of Terpenes Efficacy Isolated from Zingiber Officinale Roscoe, On Lipid Profiles and Some Biochemical Parameters in Alloxan-Induced Diabetic Male Albino Rats

Abstract

The current study was conducted at the AL-ameen center for research and advanced biotechnologies and the Faculty of science / department of Biology / animal house. Forty nine male albino rats belonging to the strain Sprague Dawley, species Rattus rattus, were used. They weighed (200 – 245)  g, and were divided into 7 groups, 7males in each group. The first included control group that was orally dosed with a solution of physiological salt 0.9% NaCl. The second group was injected subcutaneously with alloxan 100 mg/kg. The third and fourth groups were orally administrated with terpene extract at the two concentrations 150 and 300 mg/kg respectively. The fifth and sixth groups were injected with alloxan 100 mg/kg, then was orally subjected to terpene extract 150 and 300 mg/kg respectively. The last group was orally given amaryl solution 0.1 mg/kg after being injected with alloxan 100 mg/kg. It is worth mentioning that the dosage process lasted for 30 days, once per day. Regarding the results of the present study, the levels of biochemical parameters including blood glucose, total cholesterol(TC),triglycerides (TG), low-density and very low-density lipoprotein (LDL, VLDL), urea and creatinine, in addition to the liver enzymes activity of AST, ALT and ALP all showed a significant increase (P˂ 0.05) at the treated group with alloxan and the amaryl group, on the contrary, the levels of high-density lipoprotein (HDL) revealed significant decrease(P˂ 0.05)  in the same two groups, while the groups that were dosed with the terpene extract in two concentrations (150 and 300) mg /kg  revealed notable decrement (P˂ 0.05 ) in the levels of total cholesterol (TC), triglycerides (TG), and low-density and very low-density lipoprotein (LDL, VLDL), in addition to the liver enzymes activity of AST, ALT and ALP,  also levels of urea and creatinine, contrastively, the levels of HDL showed a observable elevation (P˂ 0.05) in the group that administrated with terpene at concentration of 300 mg ⁄ kg  when compared with the control group and other experimental groups.