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Burkholderia cepacia has an inherent ability to decrease the activity of antibiotics. This study aimed to screen tetracyclines and diagnose genes among B.cepacia isolated from different sources using Polymerase Chain Reaction (PCR). the results showed that among 950 specimens, 31 isolates were identified as B. cepacia isolates, and 700 of specimens exhibit bacterial growth compared with 250 no bacterial growth. Results were showed 380 return to female and 320 was return to male. The results showed a high level of resistance to each of the antibiotics ceftazidine (CAZ), trimethoprim /sulfamethoxazole (SXT) and meropenem (MEM), 27 (87%), 26 (83.87%), and 24 (77.41%) respectively. While minocycline (MIN) showed high efficacy against bacteria, with a resistance rate of 6 (19.35%). Results of PCR amplification showed high spreading of Tet- A and Tet-B among B. cepecia isolates which reached 24/31(77.41%) respectively, while tet-D and tet-E were absent among B. cepecia isolates in this study.
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