EVALUATION OF ANTI-DIABETIC AND ANTI-HYPERLIPIDEMIC ACTIVITY OF PRUNUSDULCIS SEED EXTRACT IN STREPTOZOTOCIN INDUCED DIABETIC RAT

Abstract

Amygdaline is naturally occurring chemical compound, it also known as laetrile or vitamin B17 Best known for being falsely promoted as a cancer cure. Amygdaline is a bitter substance and most found in the seed of apples, apricots, peaches, bitter almond and plums. In Indian cultural system of medicine the herbal remedies are prescribed for the treatment of various diseases including diabetes mellitus. In recent year plant are being effectively tried in a variety of pathophysiological state, Bitter Almond is one of them. PDWA extract is prepared by sox halation extraction process with 50% of ethanol and characterized by High Performance Thin Layer Chromatography and Infra Red Spectroscopy. Non insulin dependent diabetes mellitus was induced in Wistar albino rats by intraperitoneal administration of streptozotocin (60mg/kg). At the end of experiment period of 21 days reduction in the fasting blood glucose level, serum insulin, serum lipid parameter and renal function biomarker were estimated in the controlled and treated rats. Histopathological examination of liver, kidney, and pancreas were also carried out.PDWA extract were given (250mg/kg and 500mg/kg) orally for the duration of 21 days as per protocol of treatment. Blood glucose level and various biochemical parameters were measured by glucometer and respective diagnostic kit. Such as cholesterol, triglyceride, LDL, HDL, VLDL and antioxidant parameter by using diagnostic kitsOn administration of PDWA seed extract, studied blood glucose level of animal showed a significant decrease (P<0.001) in elevated blood glucose level along with biochemical parameter it shows significant to antihyperlipidemic activity and Antioxidant effects. The result showed promising effect of PDWA seed extract treatment as compared to treatment with the standard drug Metformin for Antidiabetic.