Phytochemical and Pharmacological Evaluation of Extract of Morinda Citrifolia as Anti-Inflammatory Agent

Abstract

Development of effective and economical nonsteroidal anti-inflammatory drugs (NSAIDs) with minimal or no gastrointestinal (GI) side effects is an area of importance in drug discovery pharmaceutical industry. Due to these side effects and health problems of existing anti-inflammatory therapies, natural anti-inflammatory supplements are becoming more popular and many scientific investigations have been concentrated on this area. In recent years, enormous deal of effort has focused on using available experimental techniques to verify natural antioxidant and anti-inflammatory drugs from natural product resources. Morinda citrifolia (Family: Rubiaceae) have been used since ancient times in the traditional medicinal systems like, Ayurveda, Siddha, Chinese and many other system of medicines in the treatment of various ailments. The fruits have been used topically in various conditions like, sprains, swellings, wounds and bruises. In present work Phytochemical and Pharmacological Evaluation of various Extract of Morinda Citrifolia fruit and stem were performed t proves as Anti-Inflammatory Agent. The In vitro bovine serum albumin denaturation inhibition assay of petroleum ether, chloroform, ethyl acetate, methanol and water extract of M citrifolia fruit, stem were performed. The result of M. citrifolia fruit extract (400 µg/ml) has more effect than M. citrifolia stem extract. The In vitro inhibitory effects of petroleum ether, chloroform, ethyl acetate, and methanol and water extract of both plant parts at different concentrations were measured on in-vitro enzymatic activities against COX-1 and COX-2 receptor. Methanol extract of M. citrifolia fruit extract was found more effective in all in vitro anti-inflammatory activity than other extract.