PHARMACOLOGICAL AND PHYTOCHEMICAL EVALUATION OF HEPATOPROTECTIVE POTENTIAL OF CARALLUMA UMBELLATA

Abstract

The aim of this study is to investigate the detoxification and metabolism of pharmaceuticals and other xenobiotics are carried out by the liver, an important organ. Through pharmacological and phytochemical investigation, this study aims to assess the hepatoprotective properties of Caralluma umbellata. To prepare the sample, one kilogram of Caralluma umbellata leaves were crushed into coarse powder and defatted using Soxhlet's extractor with petroleum ether (65°-85°C). Three milliliters of ethanol were mixed with 150 microliters of DPPH, and the absorbance was measured at 516 nm for the control reading. Test samples with concentrations of 50 g/ml, 100 g/ml, 250 g/ml, 500 g/ml, and 1000 g/ml were obtained. The samples were kept in a dark area for 15 minutes, and after that, the optical density was measured at 516 nm with ascorbic acid serving as the standard and methanol. These results provide that Ethanolic extract (EE) of Caralluma umbellate showed the most efficient hepatoprotective activity against PCM on HepG2 cell lines with silymarin as control compared to Caralluma umbellate extract. bioactive compounds in EE of Caralluma umbellataare further required to evaluate its potential as a potent hepatoprotective agent for liver drug formulation. Further in-vivo and clinical studies are also required to confirm its therapeutic efficacy.